cDNA sequence of the capsid protein gene and 3′ Untranslated region of a fanleaf isolate of grapevine fanleaf virus
نویسندگان
چکیده
منابع مشابه
Isolation of the Gene Coding for Movement Protein from Grapevine Fanleaf Virus
A pair of degenerate primers, GMPF1 and GMPR1, was designed on the basis of alignment of previously reported Grapevine fanleaf virus (GFLV) movement protein (MP) nucleotide sequences from Iran and other parts of the world. cDNA was synthesized by the use of Oligo d(T)18 from total RNA extraction from each diseased grapevine leaf sample and subjected to polymerase chain reaction (PCR) with the d...
متن کاملCharacterization of the Full Length Coat Protein Gene of Iranian Grapevine fanleaf virus isolates, genetic variation and phylogenetic analysis
The full-length coat protein gene of Grapevine fanleaf virus (GFLV) isolates from Iran was characterized byreverse transcription polymerase chain reaction (RTPCR) and sequencing. The expected 1515 bp coatprotein (CP) gene amplicon was obtained for 16 isolates out of 89 that were identified by double antibodysandwich enzyme-linked immunesorbent assay (DASELISA) in a population ...
متن کاملPhylogenetic status of Grapevine fanleaf virus isolate of Lorestan province of Iran
Pakbaz S, Darvishnia M, Naghavi A (2022) Phylogenetic status of Grapevine fanleaf virus isolate of Lorestan province of Iran. Plant Pathology Science 11(1):1-12. Doi: 10.2982/PPS.11.1.1 Introduction: Grapevine fanleaf virus (GFLV) is a major grapevine infecting virus in the world. Materials and Methods: Grapes showing GFLV signs were sampled from the suburbs of the city ...
متن کاملQuasispecies nature of the genome of Grapevine fanleaf virus.
Genetic diversity was characterized in 14 isolates of Grapevine fanleaf virus (GFLV) recovered from grapevine (Vitis vinifera). Virions were collected by immunocapture, and a 1557 bp fragment containing part of the viral coat protein gene and part of the untranslated region to its 3' side was amplified by RT-PCR. Sequence variation among isolates was characterized by restriction fragment length...
متن کاملThe Occurrence of Grapevine Fanleaf Virus in Washington State Vineyards
Grapevine degeneration in grapevines caused by Grapevine fanleaf virus (GFLV) has been documented in many viticultural regions worldwide (Andret-Link et al. 2004). It is one of the major economically important virus diseases affecting the longevity of grapevines and reducing the fruit yield and fruit quality. Infected grapevines show a range of foliar symptoms consisting of leaf deformation, ye...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1991
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/19.19.5440